Extended Data Fig. 7: Recyclability of nFeS in the conversion from I3C to I2CA confirms its catalytic behavior. | Nature Microbiology

Extended Data Fig. 7: Recyclability of nFeS in the conversion from I3C to I2CA confirms its catalytic behavior.

From: Nanozymes modulate probiotic tryptophan metabolism to prevent Salmonella infection in mammalian models

Extended Data Fig. 7: Recyclability of nFeS in the conversion from I3C to I2CA confirms its catalytic behavior.

a nFeS and Fe3O4 were reacted with I3C and I3CA, respectively, then recycled nFeS and Fe3O4 to next reaction with new weigh I3C and I3CA. b-e I3C, I3CA, and I2CA concentration at the end of five times of recycled reaction. nFeS is represented as Fe3S4 here. f Different concentrations of I3C as substrates reacted with nFeS (After 5 times recycled), the generation rate of I2CA per unit time was used to fit the Michaelis–Menten curve of enzyme kinetic reaction. g Concentration changes of I2CA in the reaction between I3C and nFeS during 240 min. h Redox potential of nFeSOx/nFeSRe and I3CA/I3C couples. i Standard redox potentials of NAD+/NADH, nFeSOx2/nFeSRe2, O2/HO2−, I3CA/I3C, O2/OH−, nFeSOx1/nFeSRe1, O2/H2O2, and O2/H2O. Ox represents the oxidation state. Re indicates the reduced state. Each experiment was repeated independently for three times with similar results. Panel a was created with BioRender.com.

Source Data

Back to article page