Extended Data Fig. 2: EcOmpA-ClbS is superior to D-serine, a small molecular inhibitor for colibactin biosynthesis, in suppressing genotoxicity induced by various pks+ E. coli.
From: Surface expression of antitoxin on engineered bacteria neutralizes genotoxic colibactin in the gut
a, a schematic of the coculture setup. b, D-serine failed to reduce the genotoxicity by DH10B pBAC-pks in HeLa cells while EcOmpA-ClbS completely suppressed the genotoxicity. c, EcOmpA-ClbS suppressed host DNA damage induce by pks+ E. coli (NC101, MIT A2 and MIT A21) and outperformed the inhibitory effect from D-serine (10 mM). D-serine was included in the exponentially growing phase of pks+ E. coli isolates as well as in the coculture with HeLa cells. The MOI is 20. Figures show representative data from three independent biological replicates (b, c). Two-sided unpaired t-test (b, c). Data are means ± SD (n = 3, b, c). Panel a created with BioRender.com.
