Extended Data Fig. 3: Surface display of ClbS in two pks+ E. coli chassis strains lower their intrinsic genotoxicity while exhibiting anti-colibactin activities against other pks+ bacteria.
From: Surface expression of antitoxin on engineered bacteria neutralizes genotoxic colibactin in the gut
a, flow cytometry analysis of DNA damage in Hela cells infected with EcN/EcN Δpks and NGF-1/NGF-1 Δpks. The MOI is 50. b, representative flow cytometry histogram for the surface display levels of OmpA-ClbS and OmpA alone in EcN and NGF-1. c, a schematic of bacteria-HeLa coculture. d, surface display of ClbS in two pks+ E. coli chassis strains lower their intrinsic genotoxicity while exhibiting anti-colibactin activities against other pks+ bacteria. Figures show representative data from three independent biological replicates (a, d). The MOI for NC101 or NC101Δpks is 20. The MOI for EcNOmpA-ClbS, EcNOmpA, NGF-1OmpA-ClbS or NGF-1OmpA is 400. Two-sided unpaired t-test (a, d). Data are means ± SD (a, d). Panel c created with BioRender.com.
