Fig. 3: Different mechanisms contribute to CSP-mediated antifungal resistance.

a, Semiquantitative spotting assays with different stress agents. Serial 5-fold dilutions of fungal cell suspensions were spotted onto YPD agar containing chemical agents as indicated. Semiquantitative data were collected by comparing relative growth properties of tested strains. b, Potential mechanisms implicated in CSP-mediated AMB resistance deduced from data shown in a. c, Gene Ontology enrichment for differentially expressed genes in rca1∆ and efg1∆ mutants compared to WT. Data were filtered with cut-off log₂FC of ±0.58. Common Gene Ontology terms of rca1∆ and efg1∆ were visualized. d, Spotting assays for CSP mutants on media containing 3 µg ml⁻¹ AMB and 5 mM VitC. e, MIC assays for AMB alone and in combination with the mitochondrial inhibitor AA. The AA concentration ranged from 0 to 1 µg ml⁻¹ (top to bottom) for each strain. Three biological replicates yielded similar results in a, d and e. Credit: icons in b, Bioicons.com under a Creative Commons license CC BY 3.0.

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