Extended Data Fig. 2: Biotransformation kinetics of phytonutrient substrates butein, isoliquiritigenin and butin.
From: Gut microbiome-mediated transformation of dietary phytonutrients is associated with health outcomes
a, Biotransformation kinetics of the phytonutrient substrates isoliquiritigenin (top) and butein (bottom), along with their corresponding products (respectively, liquiritigenin and davidigenin; and butin and butin-FcrP, the product resulting from the Fcr enzyme reaction of butin), as catalysed by E. ramulus under assay conditions using M9 minimal medium. Two initial substrate concentrations (20 µM and 200 µM) were tested in the presence of E. ramulus, heat-killed (HK) E. ramulus, E. coli K-12 or a bacteria-free medium control. b, Biotransformation kinetics of the phytonutrient substrate butin, along with its corresponding products, under assay conditions using mGAM medium. The corresponding products include butein and butin-FcrP (the product resulting from the Fcr enzyme reaction of butin). Lines represent mean and error bars represent s.d. calculated from three independent assay replicates.
