Extended Data Fig. 8: Overexpression of GALNT2 in A549 has a minimal impact on host genes.
From: Interferon-stimulated gene GALNT2 restricts respiratory virus infections
a, Scatter plot showing relative up- and down-regulated genes, identified by RNA-seq analysis of GALNT2-overexpressing A549 cells. GALNT2 expression was validated by western blots (Extended Data Fig. 4g). b, Proteomics: Volcano plot illustrates GALNT2-regulated proteins from the Stable Isotope Labeling By Amino Acids In Cell Culture (SILAC) experiment. Greater than 2-fold change in abundance upon GALNT2 overexpression and lower than 0.05 adjusted p value were set as the threshold criteria for GALNT2-regulated proteins (see method). c, Glycoproteomics: Volcano plot illustrates GALNT2-regulated O-glycosylation sites from the SILAC experiment. Greater than 2-fold increase in abundance upon GALNT2 overexpression and lower than 0.05 adjusted p value were set as the threshold criteria for GALNT2-regulated sites (see method). Pearson correlation coefficients and p-values by two-tailed test are indicated.
