Extended Data Fig. 5: Experimental specific acetate ratio between mutant 184f1 and Cba18-WT showed good agreement with the simulated ratio, derived from FBA secretion fluxes.
From: Phage resistance mutations in a marine bacterium impact biogeochemically relevant cellular processes
To compare experimental acetate concentrations to FBA acetate secretion fluxes, we simulated an exponential growth for Cba18-WT and 184f1 –as close as possible to the experimental conditions- and integrated the instantaneous acetate secretion rate over time, assuming no reuptake. Both experiment and simulation were done using D-glucose as a single C source, with key values during the experiment and growth simulation presented in Extended Data Table 1. Simulation of Cba18-WT and 184f1’s exponential growth and acetate concentration.
