Extended Data Table 1 Key values used during the growth simulation of Cba18-WT and 184f1 to assess acetate secretion

From: Phage resistance mutations in a marine bacterium impact biogeochemically relevant cellular processes

  

Cba18-WT

184f1

 

Experimental measurements

Initial experimental cell

concentration

1.00E+07

1.00E+07

cells/mL

Average cell concentration at

sampling

2.49E+08

1.88E+08

cells/mL

Doubling time

2.91

3.56

h

Specific acetate ratio

1.6 ± 0.1

 
 

FBA-derived secretion flux

2.16

2.61

mmol acetate/gDW/h

Simulation

Conversion factor

489

489

fg dry weight/cell

Initial cell concentration

1.00E+07

1.00E+07

cells/mL

Final cell concentration

2.50E+08

1.86E+08

cells/mL

Final acetate concentration

1.07

1.16

µmol/mL

Specific acetate concentration

1.58E-09

2.30E-09

µmol/cell

Specific acetate ratio

1.5

 
  1. Top: Initial and final cell concentration, as well as doubling time measured during the experimental determination of acetate concentration, and used for simulating FBA-derived acetate secretion. Middle: FBA-derived acetate secretion flux in D-glucose. Bottom: Values used to simulate acetate concentration during growth simulation.
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