Extended Data Fig. 7: The PαH of S Protein is required for PBP1a activation. | Nature Microbiology

Extended Data Fig. 7: The PαH of S Protein is required for PBP1a activation.

From: Streptococcus pneumoniae S protein activates PBP1a to regulate peptidoglycan remodelling and cell division

Extended Data Fig. 7: The PαH of S Protein is required for PBP1a activation.

(a, b) Different representations of the interface between the PαH of S Protein and the helix of GT domain of PBP1a (light green) bearing the catalytic residue (E91) highlighted in red. (a) Transparent surface and ribbon representations for both molecules. (b) Hydrophobicity surface representation. (c) Hydrophobicity surface and ribbon diagram representation for S Protein and PBP1a, respectively. (d) Ribbon diagram and hydrophobicity surface representations for S Protein and PBP1a, respectively. (e) Growth of WT, ess-∆PαH, and all PαH alanine mutant strains in C + Y medium at 37 °C. (f) Western immunoblot of whole-cell lysates of WT, ess-∆PαH, L68A and F71A strains with specific anti-S Protein antibody and anti-enolase antibody as a loading control.

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