Extended Data Table 1 Synthetic lethality and viability relationships

From: Streptococcus pneumoniae S protein activates PBP1a to regulate peptidoglycan remodelling and cell division

a

Strains

WT

∆ess

∆pbp1a

∆pbp2a

∆pbp1b

∆mpgA

0

>500

>500

0

0

∆pbp2a

>500

0

0

/

>500

∆macP

>500

0

0

>500

>500

∆gpsB

>500

0

0

>500

>500

∆cbpM

>500

>500

>500

>500

>500

b

Strains

WT

∆ess

∆PαH

L68A

F71A

L68A-F71A

∆pbp2a

>500

0

0

0

0

0

∆cbpM

>500

>500

>500

>500

>500

>500

c

Strains

WT

∆ess

pbp1aR6(A124T)

pbp1aR6(A124T) ∆ess

pbp1aR6(A124T) L68A-F71A

∆pbp2a

>500

0

>500

>500

>500

∆cbpM

>500

>500

>500

>500

>500

d

Strains

WT

∆ess

∆pbp1a

∆pbp2a

∆pgdA

∆mpgA

0

>500

>500

0

>500

∆cbpM

>500

>500

>500

>500

>500

  1. The amplicons of interests are arranged vertically while the transformed strains are listed horizontally. The results are presented as the number of colonies. Strains are indicated in bold and amplicons are in light black. All transformation experiments were performed with no added DNA as the negative control and with a ΔcbpM::kan-rpsL amplicon as the positive control. Experiments made in triplicate.
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