Extended Data Table 1 Sequences and genome location of primers used for PCR amplifications
Main tiled reactions (MHRA) | ||||||
|---|---|---|---|---|---|---|
Reaction/Tile | Forward Primer Name | Forward Primer Sequence (5’-3’) | Reverse Primer Name | Reverse Primer Sequence (5’-3’) | Amplicon (start-end) | Amplicon length |
1 | PanPV-WG-Fwd | CCAGAGGCCCACGTGGCGGCTAG | PV-1999-Rev | CAAGTTGAGGGGTATCATGGTGT | 29-1999 | 1971 |
2 | PanSabin-Seg2-Fwd* | TCTGCCCRGTKGATTAYCTC | Q8-Rev | AAGAGGGTCTCTRTTCCACAT | 1533-3565 | 2033 |
3 | PanSabin-Seg3-Fwd* | GTMAATGATCACAACCC | PanSabin-Seg3-Rev* | GTTGGAAAGTTGTACATTAG | 3275-5922 | 2648 |
4 | PV-5455-Fwd | GGRCAYCARGGWGCATAYACTG | PanPV-WG-Rev | CCGAATTAAAGAAAAATTTACCCCTACA | 5423-7498 | 2077 |
Additional reactions (MHRA) | ||||||
Reaction/Tile | Forward Primer Name | Forward Primer Sequence (5’-3’) | Reverse Primer Name | Reverse Primer Sequence (5’-3’) | Amplicon (start-end) | Amplicon length |
5 | nOPV2-1864-Fwd | AACACTCCAGGGAGTAACCAGTA | Q8-Rev | AAGAGGTCTCRTTCACAT | 1844-3564 | 1721 |
6 | Y7R-Fwd | GGTTTTGTGTCAGCITGYAAYGA | nOPV-cre-Rev | TCGATACGGTGCTTGGATTTAAATTG | 2462-4545 | 2083 |
7 | Y7R-Fwd | GGTTTTGTGTCAGCITGYAAYGA | Ari-Rev | TCAATACGGTGTTTGCTCTTGAACTG | 2462-4545 | 2083 |
8 | PanSabin-Seg3-Fwd* | GTMAATGATCACACACC | PV-5643-Rev | CWCCWGGTGARGCRTGRGTTGG | 3275-5630 | 2356 |
9 | PV-4440-Fwd | CAGTCCAAGAGGTTTGCACC | PanPV-WG-Rev | CCGAATTAAAGAAAAATTTACCCCTACA | 4439-7498 | 3060 |
10 | nOPV2-5439-Fwd | CTGGGCACCAGGGTGCATA | PanPV-WG-Rev | CCGAATTAAAGAAAAATTTACCCCTACA | 5421-7498 | 2078 |
11 | Y7R-Fwd | GGTTTTGTGTCAGCITGYAAYGA | PanPV-WG-Rev | CCGAATTAAAGAAAAATTTACCCCTACA | 2462-7498 | 5036 |
12 | PanPV-WG-Fwd | CCAGAGGCCCACGTGGCGGCTAG | Q8-Rev | AAGAGGTCTCRTTCACAT | 29-3564 | 3536 |
13 | PanSabin-Seg3-Fwd* | GTMAATGATCACACACC | PanPV-WG-Rev | CCGAATTAAAGAAAAATTTACCCCTACA | 3275-7498 | 4224 |
14 | nOPV2-2845-Fwd | CAGCAGATTGTTTTCGGTT | PanPV-WG-Rev | CCGAATTAAAGAAAAATTTACCCCTACA | 2845-7498 | 4653 |
Additional reactions (IPP) | ||||||
Reaction/Tile | Forward Primer Name | Forward Primer Sequence (5’-3’) | Reverse Primer Name | Reverse Primer Sequence (5’-3’) | Amplicon (start-end) | Amplicon length |
15 | Sab2-1472-Fwd | TCTGCCCRGTKGATTAYCTC | nOPV-4545-Rev | TCGATACGGTGCTTGGATTTAAATTG | 1533-4545 | 3013 |
16 | Sab2-4165-Fwd | GCAAAGGGACTGGAGTGGG | PanPV-WG-Rev | CCGAATTAAAGAAAAATTTACCCCTACA | 4226-7498 | 3273 |
