Extended Data Fig. 6: toxicity evaluation of CD12 in C57BL/6J mice.
From: Fungal commensal promotes intestinal repair via its secreted peptide in mice
(a) Experimental timeline: C57BL/6J mice. received five cycles of CD12 or saline over 5 days. (b) Body weight trajectories were monitored; C57BL/6J mice received daily saline (control, n = 3) or CD12 (3 or 9 mg/kg, n = 4 each) for 5 days. (c) Tissue weights between groups (using the same mouse cohorts and n numbers as in Extended Data Fig. 6b). (d) Systemic toxicity profiling, including serum biomarkers including hepatic (ALT, AST), pancreatic (AMY), renal (BUN, CREA), LDH, total protein (TP) and ions exhibited no dose-dependent alterations (using the same mouse cohorts and n numbers as in Extended Data Fig. 6b). (e) Body weight changes in mice receiving a higher dose of CD12 (n = 5). (f) Organ weight comparison among groups under high-dose CD12 treatment (using the same mouse cohorts and n numbers as in Extended Data Fig. 6e). (g) Systemic toxicity profile under high-dose CD12 administration (using the same mouse cohorts and n numbers as in Extended Data Fig. 6e). Data are representative of at least three biologically independent experiments. and are presented as mean ± s.d. Statistical comparisons were conducted using two-way ANOVA (b, c, e, f) unpaired two-tailed Student’s t-test (d, g). Panel a created with BioRender.com.
