Fig. 3: WGS shows that parasex produces highly recombinogenic progeny.
From: Parasex generates highly recombinant progeny in Candida albicans with increased virulence
a, Variant positions that distinguish the parental genotypes (SC5314×529L, n = 18,623) were genotyped for progeny and plotted as a proportion of the genome in single columns. b, The number of recombination events per marker is plotted in 100-kb bins for SC5314×529L progeny. c, The number of detected recombination events for individual SC5314×529L parasexual progeny is plotted for ddRAD-seq (cyan, n = 84) and WGS (magenta, n = 5) approaches. The box plots are shown as the median and the interquartile ranges (IQRs), with whiskers extending to the extreme data points. d, The tract length between recombination events was determined for SC5314×529L WGS progeny in 10-kb bins. e, Density plot of the distances between recombination events in SC5314×529L parasexual progeny identified by ddRAD-seq (cyan) and WGS (magenta) approaches. f, Variant positions that distinguish the parental genotypes (SC5314×P78042, n = 16,111) were genotyped for progeny and plotted as a proportion of the genome in single columns. n = 45. g, The number of recombination events for each of 45 SC5314×P78042 WGS progeny. h, The tract length of fragments between recombination events was determined for SC5314×P78042 progeny in 10-kb bins. i, All variants that distinguish the 529L haploid mitochondrial genomes from SC5314 are depicted as white dots. The 529L (innermost), SC5314 (outermost) and five parasexual progeny genotypes are plotted. j, Depicted are 200 kb of ChrR with the position of recombination events in the 5 SC5314×529L WGS progeny (black boxes) and repeats (purple)32. k, The distance between 1,339 random regions and genomic repeats simulated 1,000 times was compared with distances between detected recombination events and repeats in SC5314×529L WGS progeny genomes. The box plots are presented as the median and IQRs, with whiskers extending to 1.5 × IQR. *P = 8.6 × 10−8, two-sided Student’s t-test. n = 1,000.
