Extended Data Fig. 6: Motility assay of PflD-loop truncation and PilP-loop exchange. | Nature Microbiology

Extended Data Fig. 6: Motility assay of PflD-loop truncation and PilP-loop exchange.

From: Structural insights into the assembly and evolution of a complex bacterial flagellar motor

Extended Data Fig. 6: Motility assay of PflD-loop truncation and PilP-loop exchange.

a-b, Soft agar motility assay of wild type, ΔpflD, and ΔpflD complemented with PflD or various truncations of the PflD loop region. The domain organization of full-length PflD and its various truncation constructs made for soft agar motility analyses were indicated on the top. Bars show mean ± SD; dots represent individual halos (n = 6 from three experiments). Statistical significance was calculated by two-tailed unpaired Student’s t tests between wild type and each mutants.

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