Extended Data Fig. 3: Swimming velocity of Campylobacter jejuni wild type and ΔflgY mutant in media with different viscosities.
From: Structural insights into the assembly and evolution of a complex bacterial flagellar motor
Swimming velocity of wild type or ΔflgY mutant populations after growth for 24 h in BHI broth alone or BHI broth with increasing concentrations of methylcellulose 4000 (a–c), methylcellulose 400 (d–f), or Ficoll 400 (g–i). Velocities of >100 individual cells per condition were tracked, and data from three independent assays were combined. Exact sample numbers for each panel are as follows: panel a, n = 112–222; panel b, n = 148–226; panel d, n = 112–241; panel e, n = 147–244; panel g, n = 101–183; panel h, n = 100–166. a–b, d–e, g–h show distributions of swimming velocities with mean values indicated. Differences for each treatment relative to the control (BHI) were calculated by Kruskal–Wallis test with Dunn’s post hoc multiple comparisons. c, f, i, Violin plots summarizing the data from panels a and b (c), d and e (f), and g and h (i). Red bars indicate the median; grey dash bars indicate the 25th and 75th percentiles. Comparisons between wild type (grey) and ΔflgY mutant (white) at each viscosity were also calculated by Kruskal–Wallis test with Dunn’s post hoc multiple comparisons.
