Fig. 1: Schlafen proteins mediate phage defence.
a, The computational approach to identify prokaryotic homologues of Schlafen nucleases (Slfn) with predicted roles in phage defence. This search found 5,930 unique Slfn protein sequences (100% CD-HIT cut off). The Weblogo plot shows the conserved Ex4(E/D)xK motif in prokaryotic Slfn domains. b, Annotation of domains fused to Slfn in the 5,930 identified proteins. c, Experimental approach to test for immune activities of Slfn proteins. d, Immune activities of tested Slfn proteins (top to bottom) against a panel of phages that infect E. coli (left to right). Cfr, Citrobacter freundii; Eco, Escherichia coli; Eho, Enterobacter hormaechei; Elu, Enterobacter ludwigii; Pag, Pantoea agglomerans; Ror, Raoultella ornithinolytica; dsDNA, double-stranded DNA; ssDNA, single-stranded DNA; ssRNA, single-stranded RNA; λvir, virulent mutant of phage λ.
