Extended Data Fig. 4: Ig-like domain of pSlfn5 defense confers phage trigger specificity.
Cellular toxicity assay in E. coli K-12 MG1655 cells co-transformed with a plasmid expressing RorSlfn5, SfoSlfn5, or chimeric proteins with swapped Ig-like domains and a plasmid for arabinose-inducible expression of T5.142 homologs (T5.142 – T5 phage, S114 – Salmonella phage S114, DU_PP_V - Pectobacterium phage DU_PP_V, My1 - Pectobacterium phage My1, KKP_3711 - Enterobacter phage KKP_3711, vB_PagS_AAS21 - Pantoea phage vB_PagS_AAS21). Asterisk (*) indicates toxicity phenotypes. pBAD-GFP was used as a positive control for inducible protein expression. Toxicity assays were repeated independently three times with similar results, with data shown for one representative replicate.
