Extended Data Fig. 7: RCom supplementation in mice harboring a variety of baseline gut microbiota.
a, Four cohorts of SPF C57BL/6 mice were obtained from different commercial suppliers or the same supplier but in separate shipment batches. The baseline caecal microbiota were analysed by 16S rRNA sequencing. Phylogenetic composition of common bacterial taxa at the phylum level is shown. b, Alpha diversity of baseline caecal microbiota composition. c, Principal coordinate analysis (PCoA) of baseline microbiota composition using weighted UniFrac distances. d, Four cohorts of SPF C57BL/6 mice with different baseline gut microbiota were orally supplemented with RCom or PBS for 7 days. The abundance of each of the 15 species in caecal samples was determined by qPCR and normalized to 16S rRNA level. e, PCoA plot of caecal microbiota composition in RCom- and PBS-fed mice from the four cohorts. f, Metabolic modules and their encoding MAGs enriched in RCom- versus PBS-fed mice from two cohorts (baselines 1 and 2) as determined by metagenomic shotgun sequencing. MAGs were taxonomically classified according to NCBI RefSeq. g, Butyrate synthetic pathway in gut bacteria. Each circle in b–e represents an individual animal. Baseline 1: PBS, RCom, n = 6 mice per group; Baseline 2: PBS, RCom, n = 5 mice per group; Baseline 3: PBS, RCom, n = 5 mice per group; Baseline 4: PBS, RCom, n = 4 mice per group. Data shown in b and d are mean and s.e.m. Two-tailed unpaired t-test.
