Extended Data Fig. 2: Construction of the bacterial community RCom.
a, Schematic showing the isolation of RCom members from faecal samples from anti-PD-1-responsive patients. b, A neighbor-joining phylogenetic tree of the 15 bacterial strains. Genome sequencing was conducted for the isolated 10 strains. Their closest reference strain was identified by comparing to the NCBI 16S ribosomal RNA sequence database. The RCom subsets (5-mix, 10-mix-I, 10-mix-II, and 10-mix-III) tested in vivo are indicated by colored boxes. c, For comparison, 13 bacterial strains were isolated from faecal samples from non-responder patients. Most of them are enriched in non-responders. Heatmap shows their relative abundances in responders versus non-responders among anti-PD-1-treated NSCLC patients as determined by metagenomic shotgun sequencing.
