Extended Data Fig. 6: YokF colocalizes with FlhA.
From: A family of endonucleases blocks nanotube-mediated plasmid exchange
(A) flhA-sg is motile: BSB1 (WT), and flhA-sg (GV618: flhA-lin4x-sg-Kan-Pfla/che-flhF) strains were grown to the mid-logarithmic phase, spotted onto 0.3% agar LB plates, and photographed after 12 h of incubation at 37 °C. (B) YokF and FlhA colocalization analysis: BSB1-derived strain expressing FlhA-SG (GV617: flhA-lin4x-sgd-kan- Pfla/che-flhF) was treated with anti-YokF primary antibodies followed by Alexa 647-conjugated secondary antibodies and subjected to fluorescence microscopy. Shown are fluorescence images of YokF (magenta), FlhA-SG (green), their overlay (Overlay 1), and their overlay with phase contrast (grey) (Overlay 2). Arrows highlight YokF and FlhA colocalization sites. This experiment was repeated 3 time with similar results. Scale bar 1 µm.
