Fig. 3: Viral reservoir analysis 48 months post HSCT.
a, The total proviral HIV DNA in CD4+ T cells at 48 months post HSCT quantified with ddPCR, compared with HIV-seronegative (n = 1) and HIV-seropositive aviremic controls on ART (n = 25 individual experiments). b, CS-IPDA in CD4+ T cells compared with HIV-seronegative controls (n = 2) and HIV-seropositive aviremic controls on ART (n = 24 individual experiments). c, The qVOA performed in leukapheresis sample 48 months post HSCT compared with PWH with suppressed HIV RNA on long-term ART. The IUPM value for the Oslo patient was calculated on the basis of no viral outgrowth in a single qVOA experiment of 1,312 total culture wells, each assaying 50,000 CD4+ T cells, for a total of 65,600,000 CD4+ T cells. The limit of quantification is based on the IUPM value for one culture well of viral outgrowth in the same experimental conditions. As a comparator, IUPM values for 97 individuals on suppressive ART (HIV positive) were sourced from previously published articles16,17. The centre line represents the median, and whiskers represent the interquartile range. The Oslo patient is marked in red.
