Extended Data Fig. 2: Gating strategy for lymphocyte proliferation. | Nature Microbiology

Extended Data Fig. 2: Gating strategy for lymphocyte proliferation.

From: Long-term HIV-1 remission achieved through allogeneic haematopoietic stem cell transplant from a CCR5Δ32/Δ32 sibling donor

Extended Data Fig. 2: Gating strategy for lymphocyte proliferation.

Gating strategy for the lymphocyte proliferation assay. Live cells were gated as the negative population in a dead cell stain. Single cells were gated in an SSC-A/SSC-H plot, while lymphocytes were gated in an SSC-A/FSC-A plot. CD3 positive cells were identified in an SSC-A/CD3-A plot. Single positive CD4 and CD8 cells were gated using a CD8-A/CD4-A plot. The percentage of proliferating cells was assessed in an SSC-A/CTV-A plot for both CD4 and CD8 single positive cells. CTV-A: CellTrace™ Violet–Area. Comp: compensation. FSC-A: forward scatter area. FL1-A: fluorescence channel 1 area. SSC-A: side scatter area. SSC-H: side scatter height.

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