Extended Data Fig. 2: Deletion of the GBT gene (ΔhomT) in the proposed homarine catabolic operon impairs growth of Cobetia sp. OBi1 on homarine.
From: Conserved pathway for homarine catabolism in environmental bacteria
(a) Growth of wild-type and ΔhomT on glucose or homarine. (b) Growth of ΔhomT plasmid complementation strains expressing wild-type HomT or RFP induced with 50 µM IPTG. (c) HomT complementation increases the growth rate of Cobetia sp. OBi1 ΔhomT on homarine relative to a control strain expressing RFP. Letter labels (b, c, and d) denote significantly different growth rates between sample groups (median test, p < 0.9). The y-axis position of letters labels corresponds to the median growth rate of each group. (d) RFP fluorescence of Cobetia sp. OBi1 ΔhomT cultures expressing RFP normalized to sample absorbance. The RFP data corresponds to the experiment in (b) and (c).
