Extended Data Fig. 7: Late-stage cholesterol intervention reduces lipid accumulation and restricts Mtb survival in lung macrophages.
a–e, Flow cytometric analysis of monocyte-derived alveolar macrophages (Mo-AMs), tissue-resident alveolar macrophages (TR-AMs), and interstitial macrophages (IMs) from Mtb-infected mouse lungs at 8 weeks post-infection. Shown are frequencies of Mtb+ cells (a), ADRP+ cells (b), surface MHC-II expression (c), NOS2+ cells (d), and TNF-α+ cells (e) among each indicated subset. Mice were treated daily with indicated drugs during 4–6 weeks post-infection (see Fig. 6a). f,g, Frequencies of ADRP+ cells (f) and surface MHC-II expression (g) among dendritic cells (DCs). DCs were obtained from mediastinal lymph nodes of mice at 8 weeks post-infection. h, Frequency of IFN-γ+, IL-2+, and TNF-α+ cells among CD4+ T cells after co-culture with PPD-pulsed DCs. DCs were obtained as in f,g. i,j, Frequencies of IFN-γ+, IL-2+, and TNF-α+ cells among CD4+ (i) or CD8+ (j) T cells. T cells were derived from mouse lungs infected with Mtb for 4 weeks, then treated with indicated drugs for 24 hours, followed by stimulation with PPD-pulsed mouse bone marrow-derived antigen presenting cells (APCs) for another 24 hours. k, Representative images showing H&E staining (upper) and immunofluorescence for CD19 (B cells), CD3 (T cells), and DAPI (nuclei) (lower) in mouse lungs at 8 weeks post-infection. Arrows indicate MGCs; dashed lines indicate TLSs. Boxed areas show representative regions in which MGCs or TLSs are present (Mtb-infected lungs) or absent (uninfected control lungs). l,m, Quantitation of MGCs (l) and TLSs (m) in mouse lungs at 8 weeks post-infection. Data are shown as mean ± SEM of n = 3 mice per group in a–j and 5 mice per group in l–m (two-way ANOVA with Dunnett’s post-hoc test for a–e; one-way ANOVA with Dunnett’s post-hoc test for f–j,l,m).
