Extended Data Fig. 5: TEM characterization of S-R1-R2-H and Origami-R1-R2.
From: DNA nanodevice for analysis of force-activated protein extension and interactions
a) an AlphaFold model (left; SNAP-tag: green, R1-R2: cyan, HaloTag: red) and an electron micrograph of S-R1-R2-H. b) protein end-to-end distances (Figs. 3c, 3d) fitted with Gaussian functions. c) and d) show Origami-R1-R2 before (5,748 auto-picked particles) and after (7,185 auto-picked particles) force application, respectively. Circles in the zoomed-out images contain protein molecules selected for averaging. Averaged images are centered on the U-frame cavity where S-R1-R2-H is expected. Particle numbers are shown for each class. Selected averaged TEM images are shown in Fig. 3. Scale bars: a. 50 nm, c. and d. 100 nm. Box sizes: c. 30 nm, d. 50 nm.
