Extended Data Fig. 6: DNAts stretched by various spring configurations.
From: DNA nanodevice for analysis of force-activated protein extension and interactions
a) DNAts (F1/2 ≈ 8.3 pN) pulled by single-spring devices examined by agarose gel electrophoresis. DNAts was mounted in a 9-nm gap and pulled by an unstructured DNA strand (max tension ≈ 6.1 pN), a weak hairpin (HP, F1/2 ≈ 8.8 pN) or a strong HP (F1/2 ≈ 12.8 pN). DNA devices with DNAts missing BHQ1 (Ctrl) and with complete DNAts before (-F) and after (+F) spring activation were electrophoresed. b) DNAts (F1/2 ≈ 8.3 pN) pulled by dual-spring devices (6-nm gap) examined by agarose gel electrophoresis. DNA devices with DNAts missing BHQ1 (Ctrl) and with complete DNAts pulled by the neither (L-/R-), left (L+/R-), right (L-/R+) or both springs (L+/R+) were electrophoresed. Spring activation via TMSD was performed at room temperature (21 °C) for samples on the left gel and at 37 °C for the right. Bar graphs in a and b show DNAts fluorescence quantified from the corresponding gel images (normalized to DNAts without BHQ1).
