Extended Data Fig. 8: Design and validation of the right DNA spring.
From: DNA nanodevice for analysis of force-activated protein extension and interactions
a) Schematics showing the formation of a tension-loaded (that is, extended) right spring and its activation. HP: hairpin strand with a 12-bp stem (75% GC content) and a 7-nt loop; TR: target strand that binds to HP to form tension-loaded spring; HD: displacement strand that triggers HP folding. Minimum free energy (MFE) structures are predicted by NUPACK (25 °C, 100 mM Na+ and 12 mM Mg2+). Base pair mismatches are designed to lower energy barriers for HP/TR binding and TMSD. b) Validation of reactions shown in a by non-denaturing PAGE.
