Extended Data Fig. 10: Effect of Y27632 on intracellular filamin A-talin colocalization.
From: DNA nanodevice for analysis of force-activated protein extension and interactions
Cells were seeded on fibronectin (10 µg/mL) for 8 h and treated with DMSO (control) and 10 µM of Y27632 (ROCK inhibitor) for 15 min, washed and fixed. Top: Representative images of cells treated with DMSO (left) and Y27632 (right). Immuno-fluorescence of filamin A and talin are pseudo-colored cyan and magenta, respectively. Images are 92 by 92 µm2. Below: Quantification of the fraction of FA-localized filamin A (left), FA size (middle), and filamin A to talin ratio at FA (right) in cells treated with DMSO and Y27632. Group means were compared using one-way ANOVA (n = 78 DMSO-treated cells, n = 54 inhibitor-treated cells). Data are presented as mean ± s.d.
