Extended Data Fig. 1: DMHLEPR neuron photometry responses are greater with pellet drops than with gut-infusions of caloric substances and inhibiting DMHLEPR neurons does not affect food intake following an overnight fast.
From: Food cue regulation of AGRP hunger neurons guides learning
a, Heat maps of additional well-trained mice included in Fig. 1d of DMHLEPR neuron fibre photometry responses to cue (light) onset. Each heat map represents a single behavioural session. Single trials have been sorted by response time. Green line indicates light onset (time = 0), black line indicates time when the mouse poked within the correct port. b, Heat maps of all naive mice included in Fig. 1d of DMHLEPR neuron fibre photometry responses to cue (light) onset. Each heat map represents a single behavioural session. Single trials have been sorted by response time. Green line indicates light onset (time = 0), black line indicates time when the mouse poked within the correct port. It should be noted that naive mice perform significantly fewer trials than experienced mice. c, Gastric infusion experimental design. d–f, Plot of fibre photometry responses from DMHLEPR neurons aligned to gut infusions of Ensure (d, e), saline (d, e), or caloric and non-caloric sweeteners (f). These were also aligned to food pellet drops post-infusion. Mice were either food deprived or fed ad libitum for sated Ensure infusions. Light colour tones indicate S.E.M. g, Quantification of fluorescence changes ten minutes post-gut infusions of saline, Ensure, and Ensure when animals were fed ad libitum (sated Ensure). n = 9 (mice); Repeated measures one-way ANOVA, ***P = 0.0007. h, Comparison between peak fibre photometry responses during the last 30 s of Ensure infusion (red) and first 30 s of pellet drop response (beige) when animals were given saline infusions. Peak response is significantly larger with pellet drops vs. Ensure infusions. n = 9 (animals); Two-tailed, paired t-test, *P = 0.0241. i, hM4Di-mediated inhibition of DMHLEPR neurons does not affect food intake in a post-fast refeeding assay. n = 12 (mice). Two-way ANOVA, P = 0.9992. All data represents the mean ± S.E.M.
