Extended Data Fig. 2: A time course of mTEC differentiation captured by scRNA-seq reveals expression of cell cycle regulators.
From: An alternative cell cycle coordinates multiciliated cell differentiation
a, Integrated scRNA-seq dataset of mTECs on day 1, 3, 9, and 36 at air-liquid interface culture. Clusters are distinguished by color. b, Individual day 1, 3, 9, and 36 datasets that contribute to the integrated dataset. c, Expression of select marker genes for basal stem cells (Krt5), secretory cells (Scgb3a1), deuterosome-producing differentiating multiciliated cells (Deup1) and mature multiciliated cells (Dnah5) overlaid on the UMAP of the integrated dataset. Color indicates expression level. d, Pseudotime values for proliferating basal stem cells. e, Pseudotime values for differentiating multiciliated cells. f, Heatmap of average expression of select cell cycle-related genes in both basal stem (above) and multiciliated (below) cells across S and G2/M-binned phases of the cell cycle. Color indicates expression (z-score).
