Extended Data Fig. 2: Proximity labeling reveals diversity of phagosomes containing different microbes.
From: Profiling phagosome proteins identifies PD-L1 as a fungal-binding receptor
a, Venn diagram comparing the number of phagosomal proteins identified by APEX2-based phagoPL in this study with that in two reports employing density gradient fractionation of bead-containing phagosomes. b, Venn diagram showing the number of proteins identified in each of the microbe-containing phagosomes by APEX2-based phagoPL. c, 3-axis visualization of the relative enrichment of APEX2-labeld proteins towards each microbe-containing phagosome. For each protein the log2 relative representation in the three types of phagosomes was calculated from the average intensities and the vectors along 3 axes were summed to visualize the relative biases of the proteins for the different types of phagosomes. d, Box plots showing the MS intensity of representative proteins in phagosomes containing different microbes. Box plots show the median (center line) with minimum and maximum. Data are normalized to the total protein intensity of each sample. n = 3 biological replicates. EC, E. coli; SA, S. aureus; SC, S. cerevisiae. P values by one-way ANOVA with Tukey’s multiple comparisons tests (d).
