Extended Data Fig. 4: Persistent effects of immune imprinting in the serum of mice receiving heterologous mRNA vaccines.
From: Imprinting of serum neutralizing antibodies by Wuhan-1 mRNA vaccines
a, Scheme of immunization regimen and blood draw. Mice were immunized one month apart and boosted with a third dose of mRNA vaccine three months later (n = 10, two experiments, also in Fig. 1e). Sera were collected 15 weeks after the last dose. 1273, mRNA-1273; 529, mRNA-1273.529. b, Antibody binding of sera pre-cleared with empty beads (no Dp), BA.1 spike-loaded beads (BA.1 Dp), or Wuhan-1 spike-loaded beads (Wuhan-1 Dp) to Wuhan-1 (left) and BA.1 (right) spike proteins (connecting lines represent sera from the same mouse; dotted lines show the LOD; values at the LOD are plotted slightly below the LOD for visualization). c, Percentages of Wuhan-1 (left) and BA.1 (right) spike-specific antibodies (horizontal lines and numbers at the top denote median values). Pie charts illustrate the fraction of type-specific and cross-reactive IgG in each group. d, e, Paired analysis of 3- and 15-week post-boost undepleted sera (d, 15-week data also shown in b “no Dp”) and type-specific (e, 15-week data also shown in b “BA.1 Dp” or “Wuhan-1 Dp”) antibody reactivity against Wuhan-1 (left) and BA.1 (right) spike proteins (numbers at the top indicate GMT fold reduction). c, Kruskal-Wallis ANOVA with Dunn’s post-test (P values are shown).
