Extended Data Fig. 2: Pairwise comparison of 16S rRNA sequence identity and phylogeny and pairwise comparison of Methanomethylicia MAGs.
From: Cultivation and visualization of a methanogen of the phylum Thermoproteota
A. Pairwise percent sequence identity was calculated with BLASTn. B. Maximum likelihood phylogenetic tree of full length and partial 16S rRNA genes encoded in strain LCB70 and representative Methanomethylicia MAGs. Numbers at the nodes indicate bootstrap support, only values above 90 are shown. C. Pairwise average amino acid identity of MAGs (AAI, top) and average nucleotide identity (ANI, bottom) values (in %) were calculated by compareM and FastANI, respectively. Two MAGs from the closely related class Culexarchaeia31, were used as an outgroup. The two Verstraetearchaeota-affiliated MAGs reconstructed from the original hot spring (LCB070 bin14 and bin 23) have less than 75% ANI similarity to the genome of Ca. M. verstraetei strain LCB70.
