Extended Data Fig. 4: Growth of culture LCB70 on different carbon substrates and genomic context of putative lactate dehydrogenase and heterodisulfide reductase (HdrD) subunits.
From: Cultivation and visualization of a methanogen of the phylum Thermoproteota
A. Growth of culture LCB70 on 50 mM methanol (MeOH) and 50% hydrogen (H2) in the presence or absence of L-lactate (10 mM). Data symbols for methane are the mean (n = 3 biological replicates) ± s.d. Where not visible, the error bars are smaller than the symbol. B. Growth of culture LCB70 on 50 mM MeOH and 50% H2 with 10 mM of either lactate, pyruvate, or acetate. Data symbols for methane are the mean (n = 3 biological replicates) and error bars are one standard deviation. C. Transcriptional activity of LCB70 during growth on methanol and hydrogen with lactate, pyruvate, or acetate. Plotted are the mean ± s.d. (n = 3 biological replicates). Where not visible, the error bar is smaller than the symbol. Dashed lines represents the average TPM of all genes. D. Organization of putative lactate dehydrogenase genes, double slashes indicate the GlcD and LldG clusters are in different sections of the strain LCB70 genome. Sequences are indicated by arrows and distinct domains within the protein coding sequence are indicated by black bars. LUD, Lactate Utilization Domain.
