Extended Data Fig. 6: Analysis of the E1/E2 homodimer interface.
From: The hepatitis C virus envelope protein complex is a dimer of heterodimers
a, A single E1/E2 protomer showing the buried surface area of the E2-E2 interface of the homodimeric E1/E2 complex (left) or the Fab 2A12 epitope (right)16. The same view of the ectodomain of the E1/E2 heterodimer (8rk0) is shown with E1 in steel blue and E2 in green. b, Binding of the non-neutralizing antibody 2A12 to purified E1/E2 homodimer complex (2xE1/E2) and sE2 measured by ELISA. Data is based on two technical replicates with error bars representing standard deviation. c, Sequence logo representing amino acid conservation within genotype 1–8 for each E2-E2 contact residue (Los Alamos HCV sequence database). d, Extracted HCV E1/E2 (S52mod) with alanine mutations in the contact residues of the homodimer interface, analyzed by BN PAGE western blot.
