Fig. 3: Extraction of homotrimer cycles (crowns) from T = 1 cages by pseudosymmetrization.
From: Four-component protein nanocages designed by programmed symmetry breaking
a–c,e–g,i–k, BGL17_A32 with 1.5 (a), 2.5 (e) and 3.5 (i) repeat unit arms docked into tetrahedral (TetT = 1-4; b,c), octahedral (OctT = 1-2; f,g) and icosahedral (IcoT = 1-1; j,k) T = 1 cages. Superpositions of the 3D-reconstructed nsEM map (transparent cloud) on the cage design model (colours) are shown (b,f,j). nsEM micrographs (left) and characteristic 2D class averages (right) of the cages are also displayed (c,g,k). d,h,l, C3 (crownC3-3), C4 (crownC4-2) and C5 (crownC5-1) crowns made from pseudosymmetric heterotrimers. Left, superpositions of the 3D-reconstructed nsEM map on the crown design model (ch_A (green), ch_B (blue) and ch_C (orange)). Right, 2D class averages along threefold, fourfold and fivefold symmetry axes. The diameters of the crowns are 11 nm (C3), 20 nm (C4) and 35 nm (C5). Scale bars, 100 nm (c,g,k (left)) and 10 nm (c,g,k (right),d,h,l). See Extended Data Tables 3 and 4 for the amino acid sequences of the T = 1 cages and crowns.
