Fig. 4: nsEM characterization of designed T = 4 tetrahedral, octahedral and icosahedral protein cages.
From: Four-component protein nanocages designed by programmed symmetry breaking
a–c, Ball-and-stick models of T = 1 (left) and T = 4 (right) cages for each symmetry, defined by the Caspar and Klug nomenclature8. d–o, Four component T = 4 tetrahedral cage with 48 subunits (d–g), octahedral cage with 96 subunits (h–k) and icosahedral cage with 240 subunits (l–o). Design models of the T = 4 cages (left) and a 3D-reconstructed nsEM map (right) are shown (d,h,l). In the design models, each chain is shown in a distinct colour (ch_A (green), ch_B (blue), ch_C (orange) and ch_ho (purple)). nsEM micrographs (e,i,m), characteristic 2D class averages of nsEM (f,j,n) and superpositions of the 3D-reconstructed nsEM map (grey density) on the cage design model (colours; g,k,o) are also displayed. Scale bars, 100 nm (e,i,m) and 20 nm (f,j,n). See Extended Data Table 5 for the amino acid sequences of the T = 4 cages.
