Fig. 5: Cryo-EM characterization of T = 4 octahedral and icosahedral protein cages.
From: Four-component protein nanocages designed by programmed symmetry breaking
a–d, 3D cryo-EM map of OctT = 4-3 from different views. e–g, Overlay between the cryo-EM map (grey transparent) and the design model relaxed into the map (colours) for each substructure: C4 crown (e), the homotrimer–heterotrimer interface (f) and the homotrimer (g). h–k, 3D cryo-EM map of IcoT = 4-4 from different views. The inset (h) is an adeno-associated virus (AAV) capsid shown at the same scale for size comparison. l–n, Overlay between the cryo-EM map (continuous density) and the relaxed design model (colours) for each substructure: C5 crown (l), the homotrimer–heterotrimer interface (m) and the homotrimer (n). Both the cryo-EM map and the protein model are coloured by chain (ch_A (green), ch_B (blue), ch_C (orange) and ch_ho (purple)). See Extended Data Table 7 for cryo-EM data collection information.
