Fig. 6: Thermal stability and pH tolerance of cages and internalization of the T = 4 icosahedral cage.
From: Four-component protein nanocages designed by programmed symmetry breaking
a–d, Z-average diameter of IcoT = 4-4 and OctT = 4-3 at varying temperature (a,b) and pH (c,d), measured by DLS. e, An ASGPR binding cage was generated by fusing the ch_ho chain of IcoT = 4-4 to an ASGPR-binding protein. f,g, Schematic of the ASGPR cage (f) and internalization of the cage in HEP3B cells (g). h,i, Confocal microscope images of HEP3B cells treated without (h) and with (i) the cages. Nuclei were stained with DAPI (blue), and nanocages with anti-His–Alexa-488 are shown in green. Scale bars, 30 µm (h,i).
