Extended Data Fig. 10: Global characteristics of aberrant cell-cell communication and dysregulated TGF-β pathway in KS fetal gonads revealed by 10× Genomics scRNA-seq.
From: How the extra X chromosome impairs the development of male fetal germ cells
a, Total interaction number (left) and strength (right) of inferred cell-cell communication networks among gonadal cells from CtrlM and KS. b, Circle plot showing the differential interaction number (top) and strength (bottom) among various cell types in fetal gonads of CtrlM and KS. Red edges represent increased signals while blue edges represent decreased signals in KS. c, Heatmap showing the differential interaction number and strength among various cell types between CtrlM and KS. The colour bar at the bottom right indicates that the signalling is increased (red) or decreased (blue) in KS compared to CtrlM. d, The relative information flow of each detected pathway. Yellow fonts and teal fonts represent pathways significantly upregulated (P < 0.05) in KS and CtrlM, respectively. Two-sided paired Wilcoxon test with Benjamini–Hochberg correction. e, Circle plots showing the inferred TGF-β signalling networks. The colour and width of the edge indicate the source of the signal and the probability of communication, respectively. f, Violin plots showing the expression of representative genes belonging to the TGF-β signalling pathway in various cell types. g, Bubble plots showing the representative interactions belonging to the TGF-β signalling pathway. The colour and size of the dots represent the communication probability and P value, respectively. The lack of dots indicates a zero probability of communication. One-sided permutation test.
