Extended Data Fig. 2: Cryo-EM structural determination of DGC.
From: Native DGC structure rationalizes muscular dystrophy-causing mutations
a, Data processing workflow. Binned 2 (pixel size of 2.72 Å) and binned 1 (pixel size of 1.36 Å, grey shaded) data processing is separated via dashed line. The masks used in focused 3D classification are outlined with coloured dashed lines. Data processing in RELION and cryoSPARC is denoted by a circular inscribed “R” and “C”, respectively. See Methods for more details. b, FSC as a function of spatial frequency demonstrating the resolution of the final reconstruction of DGC. c,d, View direction distribution histogram (c) and posterior precision plot (d) show view diversity of all particles used for the final map of DGC (from cryoSPARC). e, Local resolution estimation (from cryoSPARC) using a local FSC threshold of 0.5. f, FSC coefficients as a functional of spatial frequency between model and cryo-EM density maps. The generally similar appearances between the FSC curves obtained with half maps with (red) and without (blue) model refinement indicate that the refinement of the atomic coordinates did not suffer from severe over-fitting.
