Extended Data Fig. 5: Effects of ZIP, TAT, and amiloride on EPSC frequency in primary cultured neurons.

(a) Cumulative probability graph for the inter-event interval for each condition. (b) Bar graph comparison of EPSC frequency for each condition. One-way ANOVA p = 0.011; no pairwise comparisons are significant. (c) Cumulative probability graph for the inter-event interval for each condition. (d) Bar graph comparison of EPSC frequency for each condition. One-way ANOVA p = 0.09. (e) Cumulative probability graph for the inter-event interval for each condition. (f) Bar graph comparison of EPSC frequency for each condition. One-way ANOVA p < 0.0001; control vs. TTX/amiloride p < 0.0001; control vs. TTX/amiloride/TAT p = 0.0003; TTX vs. TTX/amiloride p < 0.0001; TTX vs. TTX/amiloride/TAT p = 0.0034; TTX/amiloride vs. TTX/TAT p < 0.0001; TTX/TAT vs. TTX/TAT/amiloride p = 0.0001. Error bars are centered at the mean, ± 1 s.e.m., and full statistics are provided in Supplemental Table 1.