Extended Data Fig. 9: ZIP’s removal of AMPAR nanoclusters is dependent on endoA2.
From: Cationic peptides cause memory loss through endophilin-mediated endocytosis
(a) shRNA-mediated knockdown efficiency of endoA2 mRNA. (b) Sample STED microscopy images of Homer1, GluA1, and merged for control, TTX, TTX/ZIP, shRNA, shRNA/TTX/ZIP. (c) Schematic for hypothesized mechanism of action. TTX induces homeostatic plasticity, largely through increasing the number of nanoclusters. Cationic peptides trigger remodeling of the membrane through endoA2-mediated endocytosis, which is activated only upon cationic peptide-mediated stimulation. This preferentially removes newly inserted AMPAR nanoclusters.
