Extended Data Fig. 7: The role of electron donor and co-substrate for catalysis.
From: A metagenomic ‘dark matter’ enzyme catalyses oxidative cellulose conversion
(a) The role of an electron donor (ASC) in product release under aerobic conditions for CelOCE activity. (b) Cellobionic acid production by CelOCE under aerobic and anaerobic conditions in the presence of ASC as a reductant. (c) Stoichiometric ratio for co-substrate and product formation. The reactions containing 50 mM sodium acetate pH 5.0, 0.1% (w/v) Avicel, 1 mM ASC, 5 or 10 µM H2O2, and 1 µM CelOCE were incubated at 37 °C for 16 h under anaerobic conditions. For a, b and c, results are expressed as mean ± standard deviation from three independent experiments. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test (***p < 0.001).
