Extended Data Fig. 9: Cellobionic acid production and saccharification efficiency by the secretome of engineered strains.
From: A metagenomic ‘dark matter’ enzyme catalyses oxidative cellulose conversion
The secretome of the parental (Br_TrR03) and engineered (CelOCE::Br_TrR03) T. reesei strains were assayed on technical substrates. (a, b) Cellobionic acid production using pretreated sugarcane bagasse. Saccharification efficiency on pretreated eucalyptus chips including the comparison with Cellic® CTec2 and the (LsAA9A::Br_TrR03) strain secretome (c). Cellobionic acid production using pretreated eucalyptus chips (d, e). Increased release of cellobionic acid was observed in the saccharification assays performed with the secretome of the strain co-expressing CelOCE in both technical substrates. Cellobionic acid production was monitored at 24, 48 and 72 h and the HPAEC-PAD product profiles are shown. Results from panels a, c and d are expressed as mean ± standard deviation from three independent experiments. In a and d, statistical significance was determined by one-way ANOVA with Tukey’s post hoc test (*** p < 0.001).
