Extended Data Fig. 7: Endogenous 3’-ssDNA-ends at consensus sites are associated with termination of unannotated transcripts.
From: Endogenous DNA damage at sites of terminated transcripts
a, Classification, and distribution of recurrent 3’-ssDNA-end sites relative to annotated genes. 3’-ssDNA-ends are defined as: non-template (blue; located on the sense strand within a gene), intergenic (yellow), and template (mageneta; located on the antisense strand within a gene). Arrows represent open reading frames. b, Subclassification and distribution of intergenic 3’-ssDNA-end sites. They are defined as: 3’ UTR (located on the extended sense strand of an upstream gene); 5’ UTR (located on the extended sense strand of a downstream gene); both and neither. c, Bicyclomycin treatment has little effect on DirectRNA-seq signal13 at the 3’-ssDNA-end sites, indicating Rho-independent transcript terminations82. Average profile of DirectRNA-seq signal in bicyclomycin- (BCM)-treated cells versus untreated cells.
