Extended Data Fig. 2: Cryo-EM workflow for NADPH dataset.
From: Snapshots of acyl carrier protein shuttling in human fatty acid synthase
a, Description of the data processing workflow used to determine the structures of hFASN with NADPH. b, Representative micrograph from the hFASN + NADPH dataset (representative of the 15,201 micrographs used for particle picking). Micrograph has been lowpass filtered to 10 Å. c, Representative 2D classes showing multiple views of hFASN. Classes were chosen from the 2D classification job before ab intio reconstruction. d,e, Unsharpened cryo-EM density of the modifying region of hFASN with the ACP docked at the DH or ER domain respectively, with corresponding 3D FSC curve.
