Extended Data Fig. 6: Western blotting analysis.
From: Dual regulation of mitochondrial fusion by Parkin–PINK1 and OMA1
(a) The pons/medulla, cerebellum, and liver from WT and Parkin−/− mice were analyzed by Western blotting with the indicated antibodies. The asterisk indicates non-specific bands detected by the anti-Mfn1 antibodies. (b) Quantification of band intensity (mean ± SD, n = 3). (c) Confirmation of the specificity of the anti-Mfn1 antibodies was performed. Mfn1 was knocked down in mouse embryonic fibroblasts using two distinct siRNAs and subjected to Western blotting with the specified antibodies. (d) Quantification of band intensity (mean ± SD, n = 3). (e) The tissues from WT and Oma1−/− mice were analyzed by Western blotting with the specified antibodies. For Pgam5, P indicates the precursor form, and M in the mature form. (f) Quantification of band intensity (mean ± SD, n = 3). Significance was determined using two-tailed Student’s t-test in (b, f) and one-way ANOVA with post-hoc Tukey (d): *p < 0.05, **p < 0.01, ***p < 0.001. Mice were analyzed at 6 weeks of age.
