Extended Data Fig. 7: Comparison of the metabolomic landscapes.
From: Dual regulation of mitochondrial fusion by Parkin–PINK1 and OMA1
(a) Volcano plot of metabolomic data from WT and Parkin−/−Oma1−/− pons/medulla. None of the 188 metabolites identified showed significant changes in the Parkin−/−Oma1−/− pons/medulla compared to WT, using an FDR threshold of 0.05 indicated by the dotted line (n = 4 WT and 5 Parkin−/−Oma1−/− mice). (b) Log2 fold change and FDR of each metabolite involved in the TCA cycle, energy metabolism, glycolysis/gluconeogenesis, purine/pyrimidine metabolism, and amino acids. (c) Activity of each of the nine TCA cycle enzymes: aconitase (ACO), isocitrate dehydrogenase (IDH), oxoglutarate dehydrogenase (OGDH), succinyl-CoA ligase (SUCLG), fumarase (FUMH), malate dehydrogenase (MDH), citrate synthase (CS), succinate dehydrogenase (SDH), and pyruvate dehydrogenase (PDH) in WT and Parkin−/−Oma1−/− pons/medulla (mean ± SD, n = 3 mice). (d) Oxygen consumption rates (OCRs) in WT and Parkin−/−Oma1−/− brain (mean ± SD, n = 3 mice). Mice were analyzed at 6 weeks of age.
