Extended Data Fig. 5: HSC-selective Wntless deletion does not alter liver zonation, injury and regeneration.
From: Hepatic stellate cells control liver zonation, size and functions via R-spondin 3
a. Heatmap of RNA-seq data comparing Wnt gene expression between isolated mouse HSCs and whole liver (left) and Wnt4 and Wnt5a expression in scRNA-seq from whole mouse liver. b. Wls deletion in HSCs isolated from LratCre+ Wls ΔHSC (n = 3) and LratCre- Wls fl/fl (n = 3) mice, as determined by qPCR. c. Liver-body weight ratio in LratCre+ Wls ΔHSC (n = 4) and LratCre- Wls fl/fl (n = 4) mice. d-e. CYP2E1 IHC (d) and CYP1A2 IHC (e) and quantification for Wls fl/fl (n = 10) and Wls ΔHSC (n = 11) mice f. qPCRs for Wnt target genes Cyp2e1, Cyp1a2, Lect2 and Axin2 in livers from Wls fl/fl (n = 9) and Wls ΔHSC (n = 11) mice. g. Liver injury induced by APAP (300 mg/kg) was determined by ALT measurement and quantification of the necrosis area 24 h after APAP injection in LratCre+ Wls ΔHSC (n = 7) and LratCre- Wls fl/fl (n = 6) mice. Data are shown as mean ± s.e.m. Each dot represents one biological replicate (b-g). Scale bars, 100 µm (d,e,g). P-values were calculated using unpaired two-tailed t-tests (b-g).
